Journal: bioRxiv

Article Title: RNA polymerase loss by nuclear rupture drives LMNA cardiomyopathy

doi: 10.64898/2026.04.03.716433

Figure Lengend Snippet: A) Immunofluorescence for gamma-H2AX in cardiomyocytes expressing NLS-tdTomato and GFP-icGAS isolated from mice at day 14 post tamoxifen. Texts and icons below images indicate nuclear states. Scale bar: 5 μm. B) Nuclear gamma-H2AX intensity by nuclear states. Density and box plots (interquartile range): signal distribution of all affiliated nuclei. Circles: mean intensity within individual biological replicates (color coded). Statistics: P < 0.05 (*) or P≥0.05 (N.S.) from t-tests on linear regression-estimated means with mouse-clustered standard errors. Underlying data: 164 intact nuclei from 3 WT mice, 152 intact, 116 ruptured, 50 resealed nuclei from 3 Lmna CKO mice. C) Relationship between gamma-H2AX intensity and NLS-tdTomato intensity in nuclei of Lmna CKO cardiomyocytes (340 nuclei from 3 mice). Line: simple linear regression fit with 95% confidence interval. R: Pearson’s correlation coefficient. P: t-test p -value on linear regression-estimated means with mouse-clustered standard errors.

Article Snippet: Rabbit anti-gamma-H2AX antibody (Cell signaling, 9718); mouse anti-BrU/BrdU antibody (BD Biosciences, 555627); rabbit anti-RNA Pol II N-terminal domain (NTD) antibody (Cell signaling, 14958); rat anti-RNA Pol II CTD phospho-Ser5 antibody (Chromotek, AB_2631404); rat anti-RNA Pol II CTD phospho-Ser2 antibody (Millipore Sigma, 04-1571); mouse anti-Lamin A/C antibody (Santa Cruz, sc-376248); rabbit anti-Lamin A antibody (Abcam, ab26300); rabbit anti-PCM1 antibody (Sigma, HPA023370); mouse anti-BANF1 antibody (Abnova, H00008815-M01); rabbit anti-LEMD2 antibody (Millipore Sigma, HPA017340); rabbit anti-CHMP4B antibody (Proteintech, 13683-1-AP); rabbit anti-CHMP7 antibody (Proteintech, 16424-1-AP); mouse anti-VPS4 antibody (Santa Cruz, sc-133122); and mouse anti-c-Myc antibody (Invitrogen, 13-2500).

Techniques: Immunofluorescence, Expressing, Isolation

Journal: EMBO Reports

Article Title: Inhibition of the minor spliceosome restricts the growth of a broad spectrum of cancers

doi: 10.1038/s44319-025-00511-8

Figure Lengend Snippet: ( A ) RT-PCR analysis of MIG splicing changes in A549 cells after 72 h treatment with 10 nM ASO. Schematic depictions of the obtained amplicons are shown on the right with the minor intron in red and the upstream and downstream exons coloured blue and orange, respectively. Exons not separated by a minor intron are grey. ( B ) Representative images of γH2Ax (Ser139) staining in A549 cells 72 h after 10 nM ASO treatment. Scale bar is 10 µm.

Article Snippet: IF & FACS: Rabbit monoclonal anti gamma-H2AX (Ser139) (20E3) , Cell Signaling Technologies , Cat#9718; RRID: AB_2118009.

Techniques: Reverse Transcription Polymerase Chain Reaction, Staining

Journal: Advanced Functional Materials

Article Title: Alleviation of Aging‐Related Hallmarks in a Mouse Model of Progeria via a Nanoparticle‐Based Artificial Transcription Factor

doi: 10.1002/adfm.202425944

Figure Lengend Snippet: Figure 4. Amelioration of aging-associated hallmarks in LMNA G608G cells via Oct4-nanoscript. A) Immunofluorescence for Oct4+ and H3K9me3+ cells in control and LMNAG/G fibroblasts with or without Oct4-nanoscript. Scale bar = 20 μm. B) Quantification of the fluorescence intensity of H3K9me3 in nuclei using a single confocal section. Data represent mean ± SEM. Two-way ANOVA-test, *P < 0.05, **P < 0.01; n = 12 from three samples per group. C) The relative intensity of H3K9me3 levels in control and LMNAG/G fibroblasts treated with Oct4-nanoscript. Data represent mean ± SEM. Two-way ANOVA-test, **P < 0.01; n = 3 per group. D,E) Western blot analysis of 𝛾-H2AX and total histone H3 in control and LMNAG/G fibroblasts treated with

Article Snippet: The cells were incubated overnight at 4 °C with following primary antibodies: anti-Oct4 (Abcam, ab18976; Santacruz, sc-5279), anti-H3K9me3 (Abcam, ab8898), anti-H4K20me3 (Abcam, ab78517), anti-phospho-gamma H2AX (Cell signaling, 9718), anti-Lamin B (Santacruz, sc-374015), antiLamin A/C (BD Biosciences, 612 162), anti-p16INK4A (Invitrogen, PA520379), and anti-α-SMA (Invitrogen, 14-9760-82).

Techniques: Control, Western Blot